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Image Search Results
Journal: Nature Communications
Article Title: Melanoma-specific MHC-II expression represents a tumour-autonomous phenotype and predicts response to anti-PD-1/PD-L1 therapy
doi: 10.1038/ncomms10582
Figure Lengend Snippet: Melanoma cell lines were treated with IFNγ for 24 h before collection and live-cell staining and flow cytometry analysis for MHC-I/HLA-A/B/C ( a ), MHC-II/HLA-DR ( b ) and PD-L1 ( c ). Bars represent mean±s.e.m. for at least three experiments ( d ) Representative flow plots from c . ( e ) Western blot analysis of melanoma cell lines after 24 or 48 h of IFNγ stimulation. ( f ) Phosphorylation of STAT1 (top row) and STAT5 (bottom row) in melanoma cell lines at 15 min after IFNγ stimulation. Histograms were coloured according to the arcsinh transformed ratio or MFI medians relative to the table minimum value.
Article Snippet: Flow cytometry was performed using the following antibodies: HLA-DR/BV421 (BD Horizon, clone G46-6, 1:40),
Techniques: Staining, Flow Cytometry, Western Blot, Transformation Assay