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Melanoma cell lines were treated with IFNγ for 24 h before collection and live-cell staining and flow cytometry analysis for MHC-I/HLA-A/B/C ( a ), MHC-II/HLA-DR ( b ) and PD-L1 ( c ). Bars represent mean±s.e.m. for at least three experiments ( d ) Representative flow plots from c . ( e ) Western blot analysis of melanoma cell lines after 24 or 48 h of IFNγ stimulation. ( f ) Phosphorylation of STAT1 (top row) and <t>STAT5</t> (bottom row) in melanoma cell lines at 15 min after IFNγ stimulation. Histograms were coloured according to the arcsinh transformed ratio or MFI medians relative to the table minimum value.
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Melanoma cell lines were treated with IFNγ for 24 h before collection and live-cell staining and flow cytometry analysis for MHC-I/HLA-A/B/C ( a ), MHC-II/HLA-DR ( b ) and PD-L1 ( c ). Bars represent mean±s.e.m. for at least three experiments ( d ) Representative flow plots from c . ( e ) Western blot analysis of melanoma cell lines after 24 or 48 h of IFNγ stimulation. ( f ) Phosphorylation of STAT1 (top row) and <t>STAT5</t> (bottom row) in melanoma cell lines at 15 min after IFNγ stimulation. Histograms were coloured according to the arcsinh transformed ratio or MFI medians relative to the table minimum value.
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Melanoma cell lines were treated with IFNγ for 24 h before collection and live-cell staining and flow cytometry analysis for MHC-I/HLA-A/B/C ( a ), MHC-II/HLA-DR ( b ) and PD-L1 ( c ). Bars represent mean±s.e.m. for at least three experiments ( d ) Representative flow plots from c . ( e ) Western blot analysis of melanoma cell lines after 24 or 48 h of IFNγ stimulation. ( f ) Phosphorylation of STAT1 (top row) and <t>STAT5</t> (bottom row) in melanoma cell lines at 15 min after IFNγ stimulation. Histograms were coloured according to the arcsinh transformed ratio or MFI medians relative to the table minimum value.
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Melanoma cell lines were treated with IFNγ for 24 h before collection and live-cell staining and flow cytometry analysis for MHC-I/HLA-A/B/C ( a ), MHC-II/HLA-DR ( b ) and PD-L1 ( c ). Bars represent mean±s.e.m. for at least three experiments ( d ) Representative flow plots from c . ( e ) Western blot analysis of melanoma cell lines after 24 or 48 h of IFNγ stimulation. ( f ) Phosphorylation of STAT1 (top row) and <t>STAT5</t> (bottom row) in melanoma cell lines at 15 min after IFNγ stimulation. Histograms were coloured according to the arcsinh transformed ratio or MFI medians relative to the table minimum value.
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Melanoma cell lines were treated with IFNγ for 24 h before collection and live-cell staining and flow cytometry analysis for MHC-I/HLA-A/B/C ( a ), MHC-II/HLA-DR ( b ) and PD-L1 ( c ). Bars represent mean±s.e.m. for at least three experiments ( d ) Representative flow plots from c . ( e ) Western blot analysis of melanoma cell lines after 24 or 48 h of IFNγ stimulation. ( f ) Phosphorylation of STAT1 (top row) and <t>STAT5</t> (bottom row) in melanoma cell lines at 15 min after IFNγ stimulation. Histograms were coloured according to the arcsinh transformed ratio or MFI medians relative to the table minimum value.
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Becton Dickinson anti-phospho-stat1-pe
Melanoma cell lines were treated with IFNγ for 24 h before collection and live-cell staining and flow cytometry analysis for MHC-I/HLA-A/B/C ( a ), MHC-II/HLA-DR ( b ) and PD-L1 ( c ). Bars represent mean±s.e.m. for at least three experiments ( d ) Representative flow plots from c . ( e ) Western blot analysis of melanoma cell lines after 24 or 48 h of IFNγ stimulation. ( f ) Phosphorylation of STAT1 (top row) and <t>STAT5</t> (bottom row) in melanoma cell lines at 15 min after IFNγ stimulation. Histograms were coloured according to the arcsinh transformed ratio or MFI medians relative to the table minimum value.
Anti Phospho Stat1 Pe, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher gene exp cxcl9 hs00171065 m1
Melanoma cell lines were treated with IFNγ for 24 h before collection and live-cell staining and flow cytometry analysis for MHC-I/HLA-A/B/C ( a ), MHC-II/HLA-DR ( b ) and PD-L1 ( c ). Bars represent mean±s.e.m. for at least three experiments ( d ) Representative flow plots from c . ( e ) Western blot analysis of melanoma cell lines after 24 or 48 h of IFNγ stimulation. ( f ) Phosphorylation of STAT1 (top row) and <t>STAT5</t> (bottom row) in melanoma cell lines at 15 min after IFNγ stimulation. Histograms were coloured according to the arcsinh transformed ratio or MFI medians relative to the table minimum value.
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Image Search Results


Melanoma cell lines were treated with IFNγ for 24 h before collection and live-cell staining and flow cytometry analysis for MHC-I/HLA-A/B/C ( a ), MHC-II/HLA-DR ( b ) and PD-L1 ( c ). Bars represent mean±s.e.m. for at least three experiments ( d ) Representative flow plots from c . ( e ) Western blot analysis of melanoma cell lines after 24 or 48 h of IFNγ stimulation. ( f ) Phosphorylation of STAT1 (top row) and STAT5 (bottom row) in melanoma cell lines at 15 min after IFNγ stimulation. Histograms were coloured according to the arcsinh transformed ratio or MFI medians relative to the table minimum value.

Journal: Nature Communications

Article Title: Melanoma-specific MHC-II expression represents a tumour-autonomous phenotype and predicts response to anti-PD-1/PD-L1 therapy

doi: 10.1038/ncomms10582

Figure Lengend Snippet: Melanoma cell lines were treated with IFNγ for 24 h before collection and live-cell staining and flow cytometry analysis for MHC-I/HLA-A/B/C ( a ), MHC-II/HLA-DR ( b ) and PD-L1 ( c ). Bars represent mean±s.e.m. for at least three experiments ( d ) Representative flow plots from c . ( e ) Western blot analysis of melanoma cell lines after 24 or 48 h of IFNγ stimulation. ( f ) Phosphorylation of STAT1 (top row) and STAT5 (bottom row) in melanoma cell lines at 15 min after IFNγ stimulation. Histograms were coloured according to the arcsinh transformed ratio or MFI medians relative to the table minimum value.

Article Snippet: Flow cytometry was performed using the following antibodies: HLA-DR/BV421 (BD Horizon, clone G46-6, 1:40), p-STAT5/PE-Cy7 pY694 (BD Phosflow, clone 47, 1:10) and p-STAT1/PerCP-Cy5.5 pY701(BD Phosflow, clone 4A, 1:10).

Techniques: Staining, Flow Cytometry, Western Blot, Transformation Assay